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Original Research Article | OPEN ACCESS

Methanol Extract of Polyopes lancifolius Inhibits the expression of Pro-inflammatory Mediators in LPS-stimulated BV2 Microglia Cells via Downregulation of the NF-kB Pathway

RGPT Jayasooriya1, Chang-Hee Kang1, Sung-Yong Park2, Yung Hyun Choi3, Dong-Oh Moon4, Gi-Young Kim1

1Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756; 2OTTOGI Research Institute, OTTOGI Ltd., Gyeonggi-do 431-070; 3Department of Biochemistry, College of Oriental Medicine, Dongeui University, Busan 614-054; 4Department of Biology Education, College of Education, Gyeongsan, Gyeongbuk 712-714, Republic of Korea.

For correspondence:-  Gi-Young Kim   Email: immunkim@jejunu.ac.kr   Tel:+82647543427

Received: 1 April 2011        Accepted: 11 December 2011        Published: 21 February 2012

Citation: Jayasooriya R, Kang C, Park S, Choi YH, Moon D, Kim G. Methanol Extract of Polyopes lancifolius Inhibits the expression of Pro-inflammatory Mediators in LPS-stimulated BV2 Microglia Cells via Downregulation of the NF-kB Pathway. Trop J Pharm Res 2012; 11(1):43-50 doi: 10.4314/tjpr.v11i1.6

© 2012 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: This study is aimed at identifying the anti-inflammatory mechanisms of a methanol extract of Polyopes lancifolius (MEPL) in lipopolysaccharide (LPS)-stimulated BV2 microglia cells.
Methods: The expression of mRNA and protein were investigated RT-PCR and western blot analyses in LPS-stimulated BV2 microglial cells. The level of nitric oxide (NO) production was analyzed using Griess reaction. The release of prostaglandin E2 (PGE2) and tumor necrosis factor-α (TNF-α) were determined using sandwich ELISA. NF-κB activation was detected using EMSA methods.
Results: MEPL significantly suppressed NO production in LPS-stimulated BV2 cells without any cytotoxicity. The results also indicate that MEPL decreased the production of PGE2 and TNF-α in LPS-stimulated BV2 cells. Furthermore, pretreatment with MEPL resulted in a downregulation of LPS-induced mRNA and protein expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and TNF-α. Investigation of the effect of MEPL on nuclear factor-κB (NF-κB) activity, which is a potential transcriptional factor for regulating inflammatory genes such as iNOS, COX-2 and TNF-α, showed that MEPL substantially inhibited the LPS-induced DNA-binding activity of NF-κB. MEPL also suppressed the LPS-induced degradation and phosphorylation of IκBα, and it consequently blocked p65 translocation from the cytosol to the nucleus.
Conclusion: These data show that MEPL may regulate LPS-induced NO, PGE2, and TNF-α production by suppressing NF-κB activity.

Keywords: Polyopes lancifolius, Nitric oxide, Prostaglandin E2, Tumor necrosis factor-^5;, Nuclear factor-_4;B

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